Consultation and Training

If you have any questions regarding the services of the Cellular/Molecular Core Facility, contact Sheryl White 

Cellular and Molecular Biology Core - DNA Services 

  • Construct design
  • Vector modification
  • Cloning and subcloning
  • Bacterial transformation & colony screening
  • Plasmid DNA preparation
  • Probe construction and labeling
  • Library construction and screening
  • Filter array hybridization
  • Site-directed mutagenesis
  • Polymerase chain reaction (PCR)
  • Promoter Analysis

Cellular and Molecular Biology Core - RNA Services 

  • Preparation of RNA from cells or tissue
  • RNA amplification
  • Reverse transcription (RT) to produce cDNA
  • Quantitative PCR/RT-PCR
  • RT-PCR and gel analysis of products
  • Northern blot analysis
  • Rnase protection assays
  • Differential display analysis
  • siRNA design
  • in situ RNA analysis

Cellular and Molecular Biology Core - Protein Services

  • 1 & 2D SDS-polyacrylamide gels
  • Western blot analysis
  • Nuclear, cytoplasmic and membrane protein extracts
  • Electrophoresis mobility shift ("gel shift") assay

Cellular and Molecular Biology Core - Cell Culture, Histology and Microscopy Services 

  • Zeiss PALM Microlaser dissection of samples
  • Zeiss PALM Microlaser system for sections and live cells
  • Neuroleucida Morphometrics and Microscopy
  • Culture of cell lines and primary cell cultures
  • Transient and stable transfections
  • Reporter assays: luciferase, beta-galactosidase, secreted alkaline phosphatase
  • Sectioning: paraffin or frozen
  • Histological stains: hematoxylin and eosin, cresyl violet
  • Immunohistochemical procedures: immunofluorescence, peroxidase
  • Leica Vibrotome
  • Invitrogen Countess Cell Counter