E015B -- The Yokogawa CSU-W1 spinning disk confocal system is coupled to a Nikon Eclipse NI-E upright microscope with two Andor EM CCD detectors for simultaneous two-channel imaging. Three laser lines are available, 488 nm 561 nm and 640 nm. The Yokogawa CSU-W1 has two water-immersion objectives: a Nikon Apo LWD 25X/1.1NA, and a Nikon Fluor 60X/1.0NA, both with 2mm working distance. This instrument is designed for fast image collection, with rates in excess of 50 fps. With its widened-diameter Nipkow disk, the CSU-W1 provides at least four times greater field of view than comparable systems, increasing the likelihood of capturing Ca2+ transients and other events in precious tissue, and increasing throughput. The wider disk also allows wider pinhole spacing, which decreases flare or crosstalk between pinholes, significantly improving image quality. Two detectors can be used simultaneously with emission filters for 505-545nm and 580-650nm, or 505-545nm and 665-705nm.