Genome Technologies
The Genome Technologies Core Facility at the University of Vermont Cancer Center provides a wide array of efficient, user friendly, and affordable DNA analysis services to members of the University of Vermont Cancer Center, the University of Vermont research
community, and outside institutions. The facility provides a variety of services to our users including:
- massively parallel sequencing
- qPCR (dd-PCR, rt-qPCR)
- Sanger sequencing
- Nanopore sequencing
- 10x single cell
Instrumentation (as of 02-13-2024)
Singular Genomics G4 [NEXTGEN SEQUENCER]- Illumina MiSeq [NEXTGEN SEQUENCER]
- Illumina MiniSeq [NEXTGEN SEQUENCER]
- Oxford Nanopore P2Solo [NEXTGEN LONG READ SEQUENCER]
- Oxford Nanopore MinION [NEXTGEN LONG READ SEQUENCER]
- Oxford Nanopore GridION [NEXTGEN LONG READ SEQUENCER]
- Oxford Nanopore MK1C [NEXTGEN LONG READ SEQUENCER]
- 10X Chromium Single cell controller [Single Cell GENOMICS]
- BioRad QX200 ddPCR [DIGITAL DROPLET PCR]
- ABI SeqStudio [SANGER SEQUENCER]
- ABI QuantStudio 6 Flex [RTqPCR]
- ABI 7500 Fast [RTqPCR]
- Agilent Bioanalyzer 2100 [FRAGMENT SIZE ANALYZER]
- Innovaprep CP liquid concentrator systems
- Qubit 4 Flourometer [DNA QUANTIFICATION]
- Nanodrop ND1000 [DNA QUANTIFICATION/QC]
- Biorad ChemiDoc XRS+ [GEL DOC/CHEM DOC]
- Omni-Prep High Output Bead Mill Homogenizer
- Accuris isoPURE Mini [Automated Nucleic Acid Extraction]
Citing the Genome Technologies Facility
Please
remember to cite the University of Vermont Cancer Center-Larner College
of Medicine DNA and/or Microarray (if used after July 1, 2017) and/or
Massively Parallel Sequencing Facilities in your abstracts and
publications:
- "The
automated DNA sequencing (or other molecular analyses, i.e. rtQPCR,
Molecular Imaging, etc.) was performed in Vermont Integrative Genomics
Resource DNA Facility and was supported by University of Vermont Cancer
Center, Lake Champlain Cancer
Research Organization, and the UVM Larner College of
Medicine."
- The
target preparation and array hybridization was performed in the Vermont
Integrative Genomics Resource Microarray Facility and was supported by
the University of Vermont Cancer Center, Lake Champlain Cancer Research
Organization, UVM College
of Agriculture and Life Sciences, and the UVM Larner College
of Medicine."
- "The
next-generation sequencing was performed in the Vermont Integrative
Genomics Resource Massively Parallel Sequencing Facility and was
supported by the University of Vermont Cancer Center, Lake Champlain
Cancer Research Organization, UVM College
of Agriculture and Life Sciences, and the UVM Larner College
of Medicine."