How to start at the FCSPD Facility
Fill out a New FCSPD-User form and e-mail to Roxana del Rio-Guerra
Analyzer users:
- To become an analyzer (BD LSRII and MACSQuant VYB) self-user: Schedule an introductory training. Self-users require approval by FCSPD staff.
- Analyzer self-users: Schedule an appointment (FCSPD calendar in iLab)
- Analyzers by a FCSPD operator: Discuss your flow experiment with FCSPD staff and schedule an appointment. It is the responsibility of the investigator to provide a good quality sample as well as any necessary compensation control tubes.
Aria users:
- Please read the Practical Cell Sorting guide.
- FACS sorter users: Prior to your sorting appointment investigators must meet with the FCSPD staff to discuss your flow experiment and sorting strategy. For new users we highly recommend that you schedule a design meeting prior to setting up your experiment. Staff can advise you on the best approach to get you the purity and yield that you require, can recommend inclusion of necessary controls, and can review the sort logic with you.
- Fill out and return the Sort Request Formand FCSPD Biohazard Sort Form to the Facility Manager (Roxana del Rio-Guerra) prior to running samples. Sorting will be only conducted if these forms are on place 48 hours before your scheduled sorting.