Confocal Laser Microscopy

Nikon confocalThe Nikon A1R-ER is a point-scanning confocal that contains a dual scanning system built into the scan head.  It has both a high resolution galvanometer scanner and a high speed resonance scanner. The galvanometer scanner scans a single illumination point over the image field as fast as 8 frames/sec for a 512 x 512 pixel field and can be used to acquire high spatial resolution images at up to 4096 x 4096 pixels.  The resonance scanner can be used to acquire highly temporarily resolved images at up to 15 frames/sec at 1024 x 1024 pixels resolution or 420 frames/sec in band scan acquisition mode at 512 x 32 pixels resolution.  A deconvolution module provides for Enhanced Resolution of acquired images.  Signal is detected with 2 GaAsP and 2 high sensitivity PMTs for visible lasers, emission sets for 450/50, 525/50, 600/50 (UV/GFP/RFP/Cy5) and the system is set on an inverted Ti2 stage with Perfect Focus System (PFS4) allowing for live cell work. The Ti2 is an exceptionally stable, drift-free platform with hardware-triggering capabilities and high-speed imaging applications allowing quick complete slide scanning.  This confocal is driven by the NIS-Elements software platform.

Other System Components:
•LUNV laser launch with 405nm, 445nm, 488nm, 514nm, 561nm, 633nm solid state diode lasers (all 20mW output at the fiber tip)
•Hybrid resonant and Galvano scanhead for simultaneous FRAP, photostimulation and optogenetics
•Ti2 Inverted Microscope with 25mm FOV and the Perfect Focus System 4
•Live-cell chamber – stage-top incubator for CO2, temperature and humidity control
•10x, 20x, LWD 40x oil and 60x oil Objectives
•Epifluorescence components


Papers published from the MIC:

  • Bruno, S. R., et al. (2021). "DRP1-Mediated Mitochondrial Fission Regulates Lung Epithelial Response to Allergen." Int J Mol Sci 22(20).
  • Danforth, D. R., et al. (2019). "A Nonfimbrial Adhesin of Aggregatibacter actinomycetemcomitans Mediates Biofilm Biogenesis." Infect Immun 87(1).
  • Gasek, N., et al. (2021). "Comparative immunogenicity of decellularized wild type and alpha 1,3 galactosyltransferase knockout pig lungs." Biomaterials 276: 121029.
  • Gokina, N. I., et al. (2021). "Deficiency in CD4 T Cells Leads to Enhanced Postpartum Internal Carotid Artery Vasoconstriction in Mice: The Role of Nitric Oxide." Front Physiol 12: 686429.
  • Ikeh, K. E., et al. (2021). "REV1 Inhibition Enhances Radioresistance and Autophagy." Cancers (Basel) 13(21).
    Johnson, A. C., et al. (2021). "Hippocampal network dysfunction as a mechanism of early-onset dementia after preeclampsia and eclampsia." Prog Neurobiol 199: 101938.
  • Kumar, A., et al. (2022). "Inhibition of PDIA3 in club cells attenuates osteopontin production and lung fibrosis." Thorax 77(7): 669-678.
  • Li, A., et al. (2019). "Skeletal MyBP-C isoforms tune the molecular contractility of divergent skeletal muscle systems." Proc Natl Acad Sci U S A 116(43): 21882-21892.
  • Li, Z., et al. (2020). "ACE (Angiotensin-Converting Enzyme) Inhibition Reverses Vasoconstriction and Impaired Dilation of Pial Collaterals in Chronic Hypertension." Hypertension 76(1): 226-235.
  • Munson, P. B., et al. (2019). "Exosomal miR-16-5p as a target for malignant mesothelioma." Sci Rep 9(1): 11688.
  • Palmer, B. M. and S. P. Bell (2022). "Preparing Excitable Cardiac Papillary Muscle and Cardiac Slices for Functional Analyses." Front Physiol 13: 817205.
  • Rose, J. T., et al. (2020). "Inhibition of the RUNX1-CBFbeta transcription factor complex compromises mammary epithelial cell identity: a phenotype potentially stabilized by mitotic gene bookmarking." Oncotarget 11(26): 2512-2530.
  • Sepaniac, L. A., et al. (2021). "Micronuclei in Kif18a mutant mice form stable micronuclear envelopes and do not promote tumorigenesis." J Cell Biol 220(11).
  • Taatjes D.J.; Ghule P.N., B. N. A., Lee K., DeLance N.M., Evans M.F., Weaver D.L., Deakin N., Carr F.E., Sprague B.L., Stein G.S., Stein J.L. (2022). "The Shared Core Resource as a Partner in Innovative Scientific Research: Illustration from an Academic Microscopy Imaging Center." Journal of Biomedical Techniques 33(1).

Additional publications located here.