Light Microscopy

Olympus BX50 Light Microscope with QImaging Retiga 2000R digital camera.

The Olympus BX50 Light Microscope is an upright microscope with 4X, 10X, 20X, 40X (with collar correction for coverslip thickness), and optional 60X (oil) and 100X (oil) objectives. It is fitted with Mercury epi-fluorescence and Halogen direct illumination systems and binocular infinity optics eye pieces. The microscope is equipped with filter blocks to visualize 488 (excites blue, emits green, such as FITC), 568 (excites green, emits red, such as Rhodamine), with a multi filter cube which provides 488, 568 and UV light (used for DAPI). The blank setting is used for UV and bright field. Visit for further information.

Mouse aorta pic red polarized light     Mouse aorta oil red O transmitted light     Mouse aorta Sytox Green fluorescence


Papers published from the MIC:

  • Anathy, V., et al. (2018). "Reducing protein oxidation reverses lung fibrosis." Nat Med 24(8): 1128-1135.
  • Ather, J. L., et al. (2018). "Serum Amyloid A3 is required for normal lung development and survival following influenza infection." Sci Rep 8(1): 16571.
  • Habibovic, A., et al. (2016). "DUOX1 mediates persistent epithelial EGFR activation, mucous cell metaplasia, and airway remodeling during allergic asthma." JCI Insight 1(18): e88811.
  • Hoffman, S. M., et al. (2016). "Protein disulfide isomerase-endoplasmic reticulum resident protein 57 regulates allergen-induced airways inflammation, fibrosis, and hyperresponsiveness." J Allergy Clin Immunol 137(3): 822-832 e827.
  • Hoffman, S. M., et al. (2016). "Ablation of the Thiol Transferase Glutaredoxin-1 Augments Protein S-Glutathionylation and Modulates Type 2 Inflammatory Responses and IL-17 in a House Dust Mite Model of Allergic Airway Disease in Mice." Ann Am Thorac Soc 13 Suppl 1: S97.
  • Hoffman, S. M., et al. (2016). "Ablation of Glutaredoxin-1 Modulates House Dust Mite-Induced Allergic Airways Disease in Mice." Am J Respir Cell Mol Biol 55(3): 377-386.
  • Hristova, M., et al. (2016). "Airway epithelial dual oxidase 1 mediates allergen-induced IL-33 secretion and activation of type 2 immune responses." J Allergy Clin Immunol 137(5): 1545-1556 e1511.t enhanced DUOX1 expression and IL-33 secretion might present important contributing features of allergic asthma.
  • Ko, N. L., et al. (2018). "Venoarterial communication mediates arterial wall shear stress-induced maternal uterine vascular remodeling during pregnancy." Am J Physiol Heart Circ Physiol 315(3): H709-H717.
  • Li, Z., et al. (2018). "Effect of TTC Treatment on Immunohistochemical Quantification of Collagen IV in Rat Brains after Stroke." Transl Stroke Res 9(5): 499-505.
  • Marsden, C. G., et al. (2017). "Base Excision Repair Variants in Cancer." Methods Enzymol 591: 119-157.
  • McMillan, D. H., et al. (2016). "Attenuation of lung fibrosis in mice with a clinically relevant inhibitor of glutathione-S-transferase pi." JCI Insight 1(8).
  • Platz, J., et al. (2016). "Comparative Decellularization and Recellularization of Wild-Type and Alpha 1,3 Galactosyltransferase Knockout Pig Lungs: A Model for Ex Vivo Xenogeneic Lung Bioengineering and Transplantation." Tissue Eng Part C Methods 22(8): 725-739.
  • Pothen, J. J., et al. (2016). "A Computational Model of Cellular Engraftment on Lung Scaffolds." Biores Open Access 5(1): 308-319.
  • Qian, X., et al. (2018). "IL-1/inhibitory kappaB kinase epsilon-induced glycolysis augment epithelial effector function and promote allergic airways disease." J Allergy Clin Immunol 142(2): 435-450 e410.
  • Siddesha, J. M., et al. (2016). "Effect of a chemical chaperone, tauroursodeoxycholic acid, on HDM-induced allergic airway disease." Am J Physiol Lung Cell Mol Physiol 310(11): L1243-1259.
  • van der Velden, J. L., et al. (2016). "JNK inhibition reduces lung remodeling and pulmonary fibrotic systemic markers." Clin Transl Med 5(1): 36.

Additional publications located here.

Features include:

  • Epi-fluorescence objectives (10X, 20X, 40X, 60X, 100X).
  • Phase Contrast.
  • Double and triple fluorescence staining possible.
  • Computer and software for image management and archiving.